Development of a 1,2-difluorofucoside activity-based probe for profiling GH29 fucosidases
نویسندگان
چکیده
We developed the activity-based probe YL209 that can covalently functionalize diverse GH29 ?-l-fucosidases and detect these at nanogram levels after a click reaction with biotin reporter.
منابع مشابه
In vitro and in vivo comparative and competitive activity-based protein profiling of GH29 α-l-fucosidases.
GH29 α-l-fucosidases catalyze the hydrolysis of α-l-fucosidic linkages. Deficiency in human lysosomal α-l-fucosidase (FUCA1) leads to the recessively inherited disorder, fucosidosis. Herein we describe the development of fucopyranose-configured cyclophellitol aziridines as activity-based probes (ABPs) for selective in vitro and in vivo labeling of GH29 α-l-fucosidases from bacteria, mice and ma...
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در سالهای اخیر،اختلالات کیفیت توان مهمترین موضوع می باشد که محققان زیادی را برای پیدا کردن راه حلی برای حل آن علاقه مند ساخته است.امروزه کیفیت توان در سیستم قدرت برای مراکز صنعتی،تجاری وکاربردهای بیمارستانی مسئله مهمی می باشد.مشکل ولتاژمثل شرایط افت ولتاژواضافه جریان ناشی از اتصال کوتاه مدار یا وقوع خطا در سیستم بیشتر مورد توجه می باشد. برای مطالعه افت ولتاژ واضافه جریان،محققان زیادی کار کرده ...
15 صفحه اولLive Cell Imaging and Profiling of Cysteine Cathepsin Activity Using a Quenched Activity-Based Probe.
Since protease activity is highly regulated by structural and environmental influences, the abundance of a protease often does not directly correlate with its activity. Because in most of the cases it is the activity of a protease that gives rise to its biological relevance, tools to report on this activity are of great value to the research community. Activity-based probes (ABPs) are small mol...
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ژورنال
عنوان ژورنال: Organic and Biomolecular Chemistry
سال: 2021
ISSN: ['1477-0520', '1477-0539']
DOI: https://doi.org/10.1039/d1ob00054c